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This product will be discontinued in April 2014. Alternative products are available—learn more.Close
This SYBR® Green PCR Core Reagent set contains everything you need for SYBR® Green dye–based PCR amplification and detection. Life Technologies' SYBR® Green PCR Core Reagents contains a 10-pack of AmpliTaq Gold® DNA Polymerase, AmpErase® UNG, dNTP mix with dUTP, 10X SYBR® Green I PCR buffer, and 25 mM MgCl2. Reagents provided are sufficient to perform 2000 PCR reactions of 50 µL each.
This SYBR® Green PCR Core Reagent set includes:
• Separate components for maximum flexibility in reaction setup • SYBR® Green I dye—detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase—minimizes nonspecific product formation to achieve superior performance • dUTP—significantly reduces carryover contamination when used in conjunction with uracil-DNA-glycosylase
Maximum Flexibility and Convenience Life Technologies' SYBR® Green PCR Core Reagents provide maximum flexibility at reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA-binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
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