Peroxisome proliferator-activated receptor-γ (PPARg; PPAR-γ; NR1C3) is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9- hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPAR-γ. PPAR-γ is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts and skeletal muscle. PPAR-γ plays important roles in lipid and glucose metabolism, and have been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three members were called PPAR-α, -β, and -γ. Three N-terminal isoforms, called γ1, γ2 and γ3, are known to arise by alternative splicing and promoter usage from the PPAR-γ gene. RXR is an obligate partner for PPAR.
Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human PPAR-γ 1 (3-108 aa).
This antibody specifically recognizes human PPARγ1 and γ2 and cross reacts with mouse and rat PPARγ?1 and γ2. This antibody does not recognize human PPARα and δ.
Hu, Ms, Rt
Western Blot, ELISA, Immunoprecipitation, Electrophorectic Mobility Shift Assay, Chromatin Immunoprecipitation, Immunohistochemistry
(WB, E, IP, EMSA, ChIP, IHC)
Research use only