Hepatocyte nuclear factor4-α (HNF4, HNF4a; NR2A1) is a member of the orphan nuclear receptor family. HNF4-α is expressed in liver, kidney, intestine and pancreas tissue. Mutation of HNF4-α in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4-α gene is alternatively spliced and may generate up to nine different isoforms, HNF4-α 1 through HNF4-α 9.
Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human HNF4-α (393-461 aa).
This antibody specifically recognizes human HNF4-α 1, 2, 4, 5, 7, and 8 and cross reacts with mouse and rat HNF4-α 1, 2, 4, 5, 7, and 8.
Hu, Ms, Rt
Western Blot, ELISA, Immunoprecipitation, Electrophorectic Mobility Shift Assay, Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry
(WB, E, IP, EMSA, ChIP, IHC, IF)
Research use only