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This purified mouse monoclonal antibody recognizes the human TRA-1-81 antigen.
• Validated in: immunoprecipitation, immunofluorescence and western blotting • Isotype: IgM • Clone: cl.26 • Reactivity: Human • Volume: 200 µl • Buffer: PBS, pH 7.4, containing 0.1% sodium azide
The TRA-1-81 antigen is expressed by human embryonal carcinoma (EC) cells and is associated with a polydisperse glycoprotein with a molecular weight in excess of 200,000. TRA-1-81 recognizes an antigen of similar size detected as by TRA-1-60, GCTM2, K4, and K21, and all these antibodies detect similar but distinct patterns of expression on panels of tumor-derived cell lines. TRA-1-81 and TRA-1-60 epitopes are associated with distinct molecular species. TRA-1-81, TRA-1-60, GCTM2, and K21 antibodies all recognize distinct epitopes formed by modifications of a common keratan sulfate core molecule that is characteristically expressed by human EC cells. TRA-1-81, clone cl.26, likely recognizes the carbohydrate part of the molecule as TRA-1-60 activity is enhanced by neuraminidase.
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