This purified mouse monoclonal antibody recognizes the human TRA-1-81 antigen.

Antibody Specifications:

• Validated in: immunoprecipitation, immunofluorescence and western blotting
• Isotype: IgM
• Clone: cl.26
• Reactivity: Human
• Volume: 200 µl
• Buffer: PBS, pH 7.4, containing 0.1% sodium azide

The TRA-1-81 antigen is expressed by human embryonal carcinoma (EC) cells and is associated with a polydisperse glycoprotein with a molecular weight in excess of 200,000. TRA-1-81 recognizes an antigen of similar size detected as by TRA-1-60, GCTM2, K4, and K21, and all these antibodies detect similar but distinct patterns of expression on panels of tumor-derived cell lines. TRA-1-81 and TRA-1-60 epitopes are associated with distinct molecular species. TRA-1-81, TRA-1-60, GCTM2, and K21 antibodies all recognize distinct epitopes formed by modifications of a common keratan sulfate core molecule that is characteristically expressed by human EC cells. TRA-1-81, clone cl.26, likely recognizes the carbohydrate part of the molecule as TRA-1-60 activity is enhanced by neuraminidase.