This purified rabbit anti-ZO-1 (N-term) polyclonal antibody (pAb) is specific to human, mouse, rat, and chicken ZO-1 (N-Term). The anti ZO-1 (N-term) pAb recognizes the expressed product of the TJP1 gene (Tight Junction Protein 1), also known as Tjp1. Antibodies of ZO-1 (zonula occludens 1) are useful as tight junction markers, because ZO-1 is exclusively concentrated at tight junctions and it directly binds to claudin, occludin, and JAM proteins. Rabbit anti-ZO-1 (N-term) pAb can be used in immunohistochemistry (Frozen and FFPE) and immunofluorescence.

Antibody Specifications:

• Applications: Validated applications for Rabbit anti-ZO-1 (N-term) polyclonal antibody are immunohistochemistry (Frozen and FFPE) and immunofluorescence.
• Host Species: The host species of the ZO-1 (N-term) polyclonal antibody is rabbit.
• Also Known As: ZO-1 (zona occludens protein 1) is also known as TJP1 (Tight Junction Protein).
• Reactivity: Reacts with human, canine, mouse, and rat ZO-1 (N-Term).
• Product Size: Rabbit anti-ZO-1 (N-term) polyclonal antibody is available in a 100 µg pack size.

The tight junction is a cell-cell adhesion structure that constitutes the epithelial junctional complex along with adherens junctions and desmosomes. Tight junctions create a primary barrier to the diffusion of solutes across the cellular sheet and function as a boundary between the apical and basolateral membrane domains to produce their polarization. Tight junction strands are mainly composed of claudins, occludin, and JAM. Various scaffold proteins (ZO-1, ZO-2, and ZO-3) are concentrated at the cytoplasmic surfaces of the junctional complex regions to determine the specialization and localization of junctions. The zona occludens (ZO) proteins constitute the plaque structures underlying plasma membranes together with various proteins including cingulin, symplekin, the Par-3/Par-6/atypical protein kinase C complex, ZONAB, and guanine nucleotide exchange factor-H1/Lfc. All three ZO proteins have three PDZ domains, one Src homology 3 domain, and one guanylate kinase-like homologue domain in this order from their NH2 termini, which indicates that ZO-1, ZO-2, and ZO-3 are membrane-associated guanylate kinase like homologues (MAGUKs).

A recent study showed that low levels of ZO-1 expression correlated with poor patient prognosis in breast cancer. Another study observed aberrant ZO-1 expression in synovial sarcoma samples. ZO-1 antibodies are useful as tight junction markers, because ZO-1 is exclusively concentrated at tight junctions and directly binds to claudin, occludin, and JAM proteins.