This purified Rabbit Anti-ZO-1 (Mid) Polyclonal Antibody recognizes ZO-1. The immunogen for this polyclonal antibody is a synthetic peptide in the middle region of the ZO-1 protein; therefore, the antibody specifically reacts with the mid-region of the ZO-1 protein. ZO-1 antibodies are useful as tight junction markers because ZO-1 is exclusively concentrated at tight junctions, and directly binds to claudin, occludin, and Junctional Adhesion Molecule (JAM) proteins. This rabbit anti-ZO-1 polyclonal antibody is validated for use in applications such as western blotting and immunohistochemistry.
• Applications: Validated applications for ZO-1 (Mid) Polyclonal Antibody are western blotting, immunofluorescence, and immunohistochemistry.
• Host Species and Isotype: The host species of the polyclonal antibody is rabbit.
• Reactivity: Detects canine, rat, mouse, and human ZO-1 (Mid).
• Product Size: Rabbit Anti-ZO-1 (Mid) Polyclonal Antibody is available in a 100 µg pack size.
The tight junction is a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junctions seal cells to create a primary barrier to the diffusion of solutes across the cellular sheet and also function as a boundary between the apical and basolateral membrane domains to produce their polarization. Tight junction strands are mainly composed of claudins, occludin, and JAM. Various scaffold proteins have been reported to be concentrated at the cytoplasmic surfaces of the junctional complex regions to determine the specialization and localization of junctions: ZO-1, ZO-2, and ZO-3. The zona occludins (ZO) proteins constitute the plaque structures underlying plasma membranes together with various proteins including cingulin, symplekin, Par-3 Par-6 atypical protein kinase C complex, ZONAB, and guanine nucleotide exchange factor-H1/Lfc. All three ZO proteins have three PDZ domains, one Src homology 3 domain, and one guanylate kinase-like homologue domain in this order from their NH2 termini, indicating that ZO-1, ZO-2, and ZO-3 are membrane-associated guanylate kinase-like homologues (MAGUKs). Another study observed aberrant ZO-1 expression in synovial sarcoma samples. ZO-1 antibodies are useful as tight junction markers because ZO-1 is exclusively concentrated at tight junctions, and directly binds to claudin, occludin, and JAM proteins.