This purified mouse anti-Claudin-1 monoclonal antibody (mAb) is specific to human, mouse, rat, and chicken Claudin-1. Anti-Claudin-1 recognizes the expressed product of the CLDN1 gene, also known as Cldn1. Human claudin-1, like other claudin proteins, participates in cell-cell adhesion and regulates paracellular and transcellular transport of solutes across human epithelia and endothelia. The mouse anti-Claudin-1 mAb can be used in applications such as western blotting, ELISA, immunohistochemistry (frozen), immunoprecipitation, and immunofluorescence.

Antibody Specifications:

• Applications: Validated applications for mouse anti-Claudin-1 monoclonal antibody are western blotting, ELISA, immunohistochemistry (frozen), immunoprecipitation, and immunofluorescence.
• Host Species and Isotype: The host species and isotype of the anti Claudin-1 monoclonal antibody is mouse IgG1, kappa.
• Clone ID of Monoclonal Antibody (mAb): The mouse anti Claudin-1 monoclonal antibody clone is 2H10D10.
• Reactivity: Reacts with human, canine, mouse, and rat Claudin-1 proteins.
• Product Size: Mouse anti-Claudin-1 monoclonal antibody is available in a 100 µg pack size.

The claudin (CLDN) superfamily consists of at least 18 homologous proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport, complexed with two other integral transmembrane proteins, occludin and junctional adhesion molecule. Claudins are located in both epithelial and endothelial cells of all tight junction-bearing tissues. Claudins interact directly with tight junction-specific, membrane-associated guanylate kinase homologues, ZO-1, ZO-2, and ZO-3, and indirectly with AF-6 and the myosin-binding molecule cingulin. These protein-protein interactions promote scaffolding of the tight junction transmembrane proteins and provide a link to the actin cytoskeleton for transducing regulatory signals to and from tight junctions.

Human claudin-1, like other claudin proteins, participates in cell-cell adhesion and regulates paracellular and transcellular transport of solutes across human epithelia and endothelia. Recently, a claudin-1 cDNA has been isolated from human mammary epithelial cells (HMECs). As claudin-1 expression is low or undetectable in a number of breast tumors and breast cancer cell lines, it may be implicated as a possible tumor-suppressor gene.