This purified Mouse ZO-1 Monoclonal Antibody recognizes human and canine ZO-1. Tight junctions (or zonula occludens) are selective, semi-permeable barriers in the paracellular pathway of vertebrate endothelia and epithelia. This mouse anti-ZO-1 monoclonal antibody has been validated for use in ELISA, western blotting, and immunofluorescence.

Antibody Specifications:

• Applications: Validated applications for Mouse Anti-ZO-1 Monoclonal Antibody are western blotting, ELISA and immunofluorescence.
• Host Species and Isotype: The host species and isotype of the antibody is mouse IgG1κ.
• Reactivity: Mouse Anti-ZO-1 Monoclonal Antibody reacts with human and canine ZO-1.
• Clone ID of Monoclonal Antibody (mAb): The monoclonal antibody clone is ZO1-1A12.
• Product Size: Mouse Anti-ZO-1 Monoclonal Antibody is available in a 100 µg pack size.

Tight junctions (or zonula occludens) are selective, semi-permeable barriers in the paracellular pathway of vertebrate endothelia and epithelia. Tight junctions also serve to physically separate functionally and biochemically distinct regions of the plasma membrane that exist in distinct physiological compartments. ZO-1, ZO-2 and occluding are major components of vertebrate tight junctions. ZO-1 is a peripheral (nontransmembrane) membrane protein found on the cytoplasmic leaflet of tight junctions. ZO-1 has a MW of 225 kDa and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as "motif-a" and constitutes a basis for classifying tight junctions. ZO-1 is homologous to the dlg (discs-large) protein of Drosophila and the C. elegans lin-2 gene product. Loss of dlg results in neoplastic overgrowth of imaginal discs and loss of epithelial apical/basal polarity. ZO-1 and the interacting protein ZO-2 are members of a family of putative signal transduction proteins (MAGUK) whose members contain an SH3 domain, a domain with homology to guanylate kinases, and a PDZ domain (potential mediator of protein-protein interaction), found in many synapse-associated proteins.