This mouse anti-occludin monoclonal antibody is, Alexa Fluor® 488 Conjugate is specific to human, rat, canine and mouse occludin. Occludin monoclonal antibody, Alexa Fluor® 488 conjugate is highly purified from mouse ascites by protein A chromatography, before conjugation. Validated applications for occludin monoclonal antibody, Alexa Fluor® 488 conjugate are ELISA, Western blotting, and immunofluorescence.
• Applications: Validated application for occludin monoclonal antibody, Alexa Fluor® 488 conjugate are ELISA, Western blotting, and immunofluorescence.
• Host Species and Isotype: The host species and isotype of this occludin monoclonal antibody, Alexa Fluor® 488 conjugate is mouse IgG1κ.
• Clone ID of Monoclonal Antibody (mAb): This occludin monoclonal antibody, Alexa Fluor® 488 conjugate clone is OC-3F10.
• Reactivity: Occludin monoclonal antibody, Alexa Fluor® 488 conjugate detects human, mouse, canine, and rat occludin.
• Product Size: Occludin monoclonal antibody, Alexa Fluor® 488 conjugate is available in a 100 µg pack size.
The 65 kDa occludin protein was first identified in chicken using monoclonal antibodies. The chicken occludin cDNA was subsequently cloned and sequenced, and the amino acid sequence revealed that the occludin protein is organized into five distinct domains: a short amino terminal cytoplasmic domain (domain A), two extracellular loops (domains B and D) separated by a short intracellular loop (domain C), and a long carboxy-terminal cytoplasmic tail (domain E). The C-terminal tail of occludin is required for both for its localization at tight junctions and for its direct interaction with the ZO-1 protein. One interesting feature of the occludin protein is that its amino acid sequence has not been highly conserved throughout evolution.
This fact made isolating the mammalian homologues of chicken occludin a rather difficult task. Recently, however, the sequences of the full length cDNAs encoding occludin of rat-kangaroo, human, mouse, and dog were reported. At the amino acid level, the human, murine, and canine occludin proteins are highly homologous (~ 90% identity); however, the mammalian proteins exhibit a considerable degree of divergence from the rat-kangaroo and chicken proteins. Nevertheless, the overall structural features of the occludin protein are highly conserved in all the species examined. The recent identification and cloning of the mammalian occludin protein will undoubtedly facilitate the further study of TJ organization and function.