This purified mouse anti-Hemagglutinin monoclonal antibody (mAb) is specific to HA. This mAb reacts specifically with HA-tagged fusion proteins. The HA tag sequence is derived from influenza hemagglutinin and is typically used to facilitate the detection of proteins. Tags such as HA are used in conjunction with proteins to aid in the purification, identification, and functional analysis of the tagged protein. Mouse anti-Hemagglutinin mAb can be used in applications such as western blotting and ELISA.
• Applications: Validated applications for mouse anti-Hemagglutinin monoclonal antibody are western blotting and ELISA.
• Host Species and Isotype: The host species and isotype of the antibody is mouse IgG2a.
• Monoclonal Antibody Clone: The mouse anti-Hemagglutinin monoclonal antibody clone is 5B1D10.
• Reactivity: Reacts with HA-tagged fusion proteins.
• Product Size: Mouse anti-Hemagglutinin monoclonal antibody is available in a 100 µg size.
The HA tag sequence is derived from influenza hemagglutinin and is typically used to facilitate the detection of proteins. Tags, such as HA, are used to aid in the purification, identification, and functional analysis of those tagged proteins.
Through the use of standard molecular biology techniques, a cDNA that encodes the protein of interest can be cloned, in-frame, into an expression vector containing the tag sequence. The resultant tagged protein is usually referred to as an epitope-tagged protein or a fusion protein. Currently, a variety of expression vector-containing tags are commercially available. Depending on the expression vector, the resulting fusion proteins can be expressed in a variety of organisms including bacteria, yeast, insect, and mammalian cells. Detection of a tagged protein occurs by the use of an antibody directed against that particular tag. This alleviates the need to wait for the generattion of a specific antibody against any specific protein, especially newly identified proteins. Some of the more commonly used fusion tags include: c-myc, FLAG, GFP, GST, HA, His, and MBP.