This purified mouse anti Claudin-2 monoclonal antibody (mAb) is specific to human, mouse, rat, chicken Claudin-2. Anti-Claudin-2 mAb recognizes the expressed product of the CLDN2 gene, also known as Cldn2. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts also found in the brain.
• Applications: Validated applications for mouse anti Claudin-2 monoclonal antibody are western blotting, immunohistochemistry and immunocytochemistry/immunofluorescence.
• Host Species and Isotype: The host species and isotype of the anti Cluadin-2 monoclonal antibody is mouse IgG2b.
• Monoclonal Antibody Clone: The clone of mouse anti Claudin-2 monoclonal antibody is 12H12.
• Reactivity: Detects human, mouse, rat and chicken Claudin-2.
• Product Size: Mouse anti Claudin-2 monoclonal antibody is available in a 100 µg pack size.
Tight junctions (TJs) are specialized membrane domains that seal the intercellular spaces in epithelia and endothelia and thus contribute to the permeability barrier between lumenal and interstitial compartments. Tight junctions not only separate distinct physiological compartments, but they also confer selectivity to the transepithelial flux of molecules and ions through the intercellular spaces between the epithelial cells, the so-called paracellular pathway. In addition, tight junctions limit the diffusion of lipids between the apical and basolateral plasma membrane domain. Claudin and occludin, the two types of four-transmembrane domain proteins, are the major integral membrane components of the mammalian tight junction. So far, 20 distinct claudins have been identified.
Discovered in 1998, mouse Claudin-2 has an approximate molecular weight of 22.9 kDa and is a 230 amino acid, integral membrane protein, which localizes at tight junctions. Claudin-1 and Claudin-2 are structurally related, and are 38% identical at the amino acid sequence level. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts also found in the brain.