McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hank's salts to enable use outside a CO₂ incubator.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® McCoy's 5A is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco® McCoy's 5A product made in our Grand Island facility (12330-031). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Gibco® McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco® McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.