Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment.
The complete formulation is available.

Gibco® Ham's F-12K (Kaighn's) medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
For supply chain continuity, Life Technologies manufactures Gibco® Ham's F-12K (Kaighn's) medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.

Ham's F-12K (Kaighn's) medium contains no proteins or growth factors. Ham's F-12K (Kaighn's) medium is often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) medium uses a sodium bicarbonate buffer system (2.5 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.