The RadPrime DNA Labeling System is ideal for rapid preparation (<10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA (1). The RadPrime DNA Labeling System:
- Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridization
- Yields >109 cpm/µg control DNA using [ -32 P]-dCTP
- Labels 25 ng of DNA in one reactionPerformance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a RadPrime labeling reaction.