ElectroMAX™ DH12S™ Cells, a derivative of DH10B™ are suitable for transformation by electroporation (1,2). They may be used for production of single-stranded DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries. The DH12S™ strain is endA+, which allows the production of ssDNA with less contaminating double-stranded DNA. The lacIq on the F´ marker allows for regulatable expression from the lac promoter. Unlike most E. coli hosts used in cloning genomic DNA (3), the systems that restrict DNAs containing methylated cytosine and adenine residues (mcrA, mcrB, mcrC, and mrr) have been eliminated inthe DH12S™ strain, allowing the construction of more representative genomic libraries and more efficient plasmid rescue procedures (4,5). M13KO7 helper phage is also supplied for the production of ss DNA. ElectroMAX™ DH12S™ Cells offer:
  • >1 x 1010 transformants/µg efficiency with electroporation for unsurpassed cloning results
  • A unique genotype to support production of exceptionally clean single stranded DNA
  • Stable F´ episome to eliminate the need for growth on minimal media
  • Better sequence representation eliminating mcrA, mcrBC, mrr, hsdRMS restriction systems
  • Blue/white screening of recombinant clones due to lacZΔM15
  • Ready-to-use M13KO7 helper phage for single stranded DNA production