Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/ µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.
• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for Routine Subcloning Procedures
Subcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield > 1 x 106 transformants/ µg control DNA per 50 µl reaction.

Flexible Cloning Capabilities
Subcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits:
• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 ensures increased insert stability and prevents unwanted recombination
• endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α™ competent cells support replication of M13mp vectors but do not support plaque formation

Genotype:
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.