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Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/ µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting. • Designed for general, everyday use • Contain genetic markers useful for general applications
Ideal for Routine Subcloning Procedures Subcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield > 1 x 106 transformants/ µg control DNA per 50 µl reaction.
Flexible Cloning Capabilities Subcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits: • lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal • recA1 ensures increased insert stability and prevents unwanted recombination • endA1 improves the yield and quality of plasmid DNA prepared from minipreps • DH5α™ competent cells support replication of M13mp vectors but do not support plaque formation
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