Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10⁸ cfu/ µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA.

• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Maintains plasmid DNA with minimum recombination events
• Delivers high yield plasmid preparations for downstream applications
• M13mp cloning vectors may be propagated on a lawn of DH5α-FT™, DH5αF'™, DH5αF'IQ™, JM101, or JM107 for plaque formation.

Ideal for Difficult Cloning Projects
Library Efficiency® DH5α™ Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency® DH5α™ Competent Cells have mid-range transformation efficiency (>1 x 10⁸ cfu/ µg control DNA per 100 µl reaction). The strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH10B™ an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype:
F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_k^-, m_k⁺) phoA supE44 thi-1 gyrA96 relA1 λ^-

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.