- Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
- Maintains plasmid DNA with minimum recombination events
- Delivers high yield plasmid preparations for downstream applications
- M13mp cloning vectors may be propagated on a lawn of DH5α-FT™, DH5αF'™, DH5αF'IQ™, JM101, or JM107 for plaque formation.
Library Efficiency® DH5α™ Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency® DH5α™ Competent Cells have mid-range transformation efficiency (>1 x 108 cfu/ µg control DNA per 100 µl reaction). The strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH10B™ an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.