The SuperScript® Plasmid System is designed for synthesis of double-stranded cDNA from a purified mRNA population. The cDNA is suitable for directional cloning into the pSPORT1 vector or the pCMV•SPORT6 plasmid vector for subsequent generation of cDNA libraries and use of Gateway® Technology. The SuperScript® Plasmid System:

• Results in greater cDNA yields and increased full-length cDNAs with SuperScript® II RT

• Offers a simplified primer-adapter strategy for directional cloning

• Offers one-tube format for first- and second-strand reactions, improving cDNA yields

• Fractionates cDNA using efficient and convenient pre-packed columns

• Ligates inserts into a pre-cut Not I-Sal I vector prepared to minimize background