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DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. It is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.
Application Removing DNA from RNA and protein preparations.
Specific activity Specific activity is >10,000 units/mg.
Source Purified from bovine pancreas
Performance and quality testing Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined.
Unit definition One unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.001 A260 units/min/mL of reaction mixture at 25°C.
Unit reaction conditions 0.1 M sodium acetate (pH 5.0), 5 mM MgCl2, 50 µg/mL calf thymus DNA, and enzyme in 1 mL for 10 min at 25°C.
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