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Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Life Technologies' Taq DNA Polymerase, you get:
Your choice of recombinant or native enzyme
Amplification of PCR products up to 5 kb in size
An enzyme that is licensed and qualified for PCR
Applications Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
Source Native enzyme is purified from Thermus aquaticus YT1.
Unit definition One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
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