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The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.
The FreeStyle™ MAX System provides: • An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures (Figure 1) • Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week • Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium • Straightforward protocol for production scale-up
The FreeStyle™ MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week (Figure 2). Contents and Storage: Store FreeStyle™ MAX Reagent at +4°C.
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