Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
|Concentration||30-60 U/ µL|
|Contents||Not I 10X Buffer H (1mL) 10X Loading Buffer (1mL) |
|Reaction Mixture ||Not I 1 µL 10X Buffer H 2 µL 0.10% BSA 2 µL 0.10% Trion X-100 2 µL Substrate DNA <1 µg Sterile water to 20 µL |
|Heat inactivation||Enzyme is inactivated by heating at 65°C for 20 minutes.|
|Star Activity||Unrelated site may be under conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH >8.0.|
|Effect of DNA Methylation||Enzyme activity is affected by CG methylase depending on the sequence of the recognition site or the sequence following the site.|
|Unit Definition||One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.|
|10X Buffer H||500 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 1,000 mM NaCl |
Relative Activity in Universal Reaction Buffers
|Relative Activity (%)||<20*||<20*||20**||<20||<20*|
|*Weak star activity is detected. **100% activity is obtained by addition of 0.01% BSA and 0.01% Triton® X-100.|
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.