5´-TCG↓CGA-3´ 3´-AGC↑GCT-5´
Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
| Concentration | 4-12 U/ µL |
| Contents | Nru I 10X NruI Buffer (1mL) 10X Loading Buffer (1mL) |
| Reaction Temperature | 37°C |
| Reaction Mixture | Nru I 1 µL 10X Nru I Buffer 2 µL 0.10% BSA 2 µL Substrate DNA <1 µg Sterile water to 20 µL |
| Heat inactivation | Enzyme is inactivated by heating at 65°C for 20 minutes. |
| Star Activity | |
| Effect of DNA Methylation | Enzyme activity is affected by CG methylase. When the recognition site sequence is TCGCGATC, the enzyme activity is affected by dam methylase. In this case, general DNA originated from E. coli cannot be cleaved by this enzyme. |
| Unit Definition | One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C. |
| 10X Buffer M | 100 mM Tris-HCl, pH 7.5 70 mM MgCl2 10 mM Dithiothreitol (DTT) 0.1% BSA 1500 mM KCl |
| Source | Nocardia rubra |
Relative Activity in Universal Reaction Buffers
| Buffer | L | M | H | K | T(+BSA) | NruI Buffer |
|---|
| Relative Activity (%) | 0* | <20* | 20* | 20* | <20* | 100 |
| *The relative activity in Universal Buffers is shown as % of activity in Nru I Buffer. |
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
