5´-GT↓(Py)(Pu)AC-3´ 3´-CA (Pu)(Py)↑TG-5´Important:
Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.
|Concentration||4-12 U/ µL|
|Contents||Acc I 10X Buffer M (1mL) 10X Loading Buffer (1mL) |
|Reaction Mixture ||Acc I 1 µL 10X Buffer M 2 µL Substrate DNA <1 µg Sterile water to 20 µL |
|Heat inactivation||Enzyme inactivated by heating at 80°C for 20 minutes.|
|Star Activity||Unrelated site may be cut under conditions of low ionic strength, high enzyme concentration, or glycerol concentration >5%.|
|Effect of DNA Methylation||Enzyme activity is affected by CG methylase depending on the sequence of the recognition site or the sequence following the site.|
|Unit Definition||One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.|
|10X Buffer M||100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 500 mM NaCl |
Relative Activity in Universal Reaction Buffers
|Relative Activity (%)||20||100||<20||<20*||160|
* weak star activity is detected For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.