Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. Concentrated forms of trypsin can be aseptically diluted to 1x using a balanced salt solution without calcium and magnesium. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions.

Life Technologies offers a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing

The complete formulation is available.

Quality Testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability
Life Technologies can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® Trypsin solution made in our Scotland facility (25200-056). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.