- Title:
- Features of the parkin/ariadne-like ubiquitin ligase, HHARI, that regulate its interaction with the ubiquitin-conjugating enzyme, Ubch7.
- Authors:
- Ardley HC, Tan NG, Rose SA, Markham AF, Robinson PA,
- Journal:
- J Biol Chem 2001 276(22):19640-19647
- Product Usage:
- 293T cells and COS-7 cells were grown in DMEM with GlutaMAX supplemented with 10% FBS, 100 U/mL penicillin, and 100 ug/mL streptomycin.
- ID:
- PN63128
|
- Title:
- Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis.
- Authors:
- Alvarez-Tejado M, Naranjo-Suarez S, Jiménez C, Carrera AC, Landázuri MO, del Peso L,
- Journal:
- J Biol Chem 2001; 276(25):22368-74
- Product Usage:
- Undifferentiated PC12 cells were maintained in RPMI 1640 medium with GlutaMAX supplemented with 10% horse serum, 5% FBS, 100 U/ml penicillin and 100 ug/ml streptomycin.
- ID:
- PN65765
|
- Title:
- Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.
- Authors:
- Sobolevsky Alexander I; Beck Christine; Wollmuth Lonnie P;
- Journal:
- Neuron 2002 33(1):75-85
- Product Usage:
- Xenopus laevis oocytes were prepared as described (Wollmuth et al., J. Physiol., 491:779-797. (1996) ) and were maintained in a nutrient OR-3 medium, containing 50% L-15, 50 µg/ml penicillin-streptomycin, 5 mM glutamine, and 15 mM Na-HEPES (pH 7.2, NaOH) (all GIBCO-BRL, Grand Island, NY).
- ID:
- PN60069
|
- Title:
- Fibrin inhibits peripheral nerve remyelination by regulating schwann cell differentiation.
- Authors:
- Akassoglou Katerina; Yu Wei Ming; Akpinar Pinar; Strickland Sidney;
- Journal:
- Neuron 2002 33(6)861-75
- Product Usage:
- PCR was used to amplify the 324 bp fibronectin gene. The PCR products were cloned using the TA PCR-cloning system. Cloning was performed in both orientations and accordingly sense and antisense mRNA probes were produced using the T7 promoter of the PCR 2.1 vector. Total RNA was extracted from sciatic nerves and Schwann cell cultures using Trizol. cDNA was then generated using Superscript II reverse transcriptase.
- ID:
- PN64361
|
- Title:
- Electrophoretic profiling of both RNA and protein from a single 250-pL sample.
- Authors:
- Zabzdyr Jennifer L; Lillard Sheri J;
- Journal:
- Anal Chem 2002 74(8)1857-1862
- Product Usage:
- Gibco (Invitrogen) 0.25% Trypsin-EDTA was used to remove Cho-K1 cells from cell culture flasks. Gibco (Invitrogen) penicillin/streptomycin was present in the Cho-K1 cell growth medium to prevent bacterial growth. Gibco F-12K (Kaighn's modification) is optimal for growing Cho-K1 cells.
- ID:
- PN65768
|
