Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco® DPBS require pH adjustment and filtration at the time of preparation (see protocol for details).

Life Technologies offers a variety of Gibco® DPBS formulations for a range of cell culture applications.
The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® DPBS is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco® DPBS product made in our Grand Island facility (14200-075). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.