Hank's Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

Life Technologies offers a variety of Gibco® HBSS formulations for a range of cell culture applications.
The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco® HBSS product made in our Scotland facility (14170-138). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.