Hank's Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation. Life Technologies offers a variety of Gibco® HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® HBSS is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco® HBSS product made in our Grand Island facility (14065-056). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 and ISO 9001 standards.

Concentrated forms of Gibco® HBSS require pH adjustment and filtration at the time of preparation (see protocol for details).