This purified Mouse Anti-Connexin-43 Monoclonal Antibody is specific to rat, human, mouse, connexin-43. Anti-Connexin 43 monoclonal antibody recognizes the expressed product of the GJA1 gene. Reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis. This Connexin 43 monoclonal antibody is validated for use in Western Blot, Immunohistochemistry, Immunoassay (ELISA), and Immunocytochemistry/Immunofluorescence.
• Applications: Validated applications for Connexin 43 monoclonal antibody are Western Blot, Immunohistochemistry, Immunoassay (ELISA), and Immunocytochemistry/Immunofluorescence.
• Host Species and Isotype: The host species and isotype of the Connexin 43 monoclonal antibody is mouse IgG1-κ.
• Clone ID of Monoclonal Antibody (mAb): The connexin 43 monoclonal antibody clone is CX-1B1.
• Reactivity: Detects rat, human, and mouse connexin-43
• Product Size: Mouse Anti-Connexin-43 Monoclonal Antibody is available in a 100 µg pack size.
Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Comparison of the amino acid sequences of the various connexin family members indicate that the two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.