This mouse anti-STAT3 monoclonal antibody is specific to human, mouse and rat STAT3. STAT3 monoclonal antibody recognizes the expressed product of the STAT3 gene also known as Stat3, Stat3. The 89 kDa STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNα, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Validated applications for STAT3 monoclonal antibody are Western blotting, gel-mobility shift assay, ELISA and immunoprecipitation.
Applications: validated for Western blotting, gel-mobility shift assay, ELISA and immunoprecipitation
Host species and isotype: mouse IgG1-Kappa.
Clone ID of monoclonal antibody (mAb): ST3-5G7
Reactivity: human, mouse and rat STAT3
Product size: 100 µg pack size
STATs (signal transducers and activators of transcription) were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play essential roles in interferon IFNα- and IFNγ-regulated gene expressionx. Following the identification of these first two family members, five additional mammalian STAT proteins were cloned and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6. More recently a putative Drosophila STAT protein termed D-STAT or Marelle was described.
In unstimulated cells STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, specific STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding resulting in the transcriptional activation of distinct target genes. At least one and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members. Nevertheless, a striking specificity of STAT activation is seen in response to individual cytokines.
STAT3 (or APRF- for acute phase response factor) was the first of the second series of STAT proteins to be identified. The 89 kDa STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNα, IL-10, IL-6, IL-11, onM, LIF, CNTFG-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT 3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element). These sites are variants of the GAS element found in IFN-γ inducible genes. Using repeated cycles of binding and PCR amplification reactions , the STAT3 “high-affinity” DNA consensus binding site was identified to be TCC(G=C)GGAA. The mouse Stat3 gene is found on chromosome 11 where it co-localizes together with the Stat5a/Stat5b genes. Given that the D-Stat (marelle) gene is most related to Stat5, it is thought that the Stat3-Stat5 site represents the ancestral gene site.