Mouse anti-MAP Kinase (ERK1+ERK2) Monoclonal Antibody is specific to Canine, Human, Mouse, Rat, and recombinant ERK1+2. This unconjugated Mouse anti-MAP Kinase monoclonal antibody has been validated for use in Immunohistochemistry, ELISA, Immunoprecipitation, Electrophoretic Mobility Shift Assays (EMSA) and Western Blotting. Mouse anti-ERK1/2 (MAP kinase) recognizes the expressed products of the MAPK1 and MAPK3 genes.
• Applications: Validated applications for Mouse anti-MAP Kinase (ERK1/2) Monoclonal Antibody are western blotting, ELISA, immunoprecipitation and electrophoretic mobility shift assay.
• Host Species and Isotype: The host species and isotype of the anti-MAPK (ERK1/2) antibody is mouse IgG1κ.
• Clone ID of Monoclonal Antibody (mAb): The monoclonal antibody clone is ERK-7D8.
• Reactivity: Detects human, canine, mouse, rat, and recombinant ERK1/2.
• Product Size: Mouse Anti-MAP Kinase (ERK1/2) Monoclonal Antibody is available in a 200 µg pack size.
Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family is the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggests that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including RSK, c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK. MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by multiple factors.