This mouse anti-STAT5 Beta monoclonal antibody is specific to human, mouse and rat STAT5 Beta. STAT5 Beta monoclonal antibody recognizes the expressed product of the STAT5B gene. STATs (signal transducers and activators of transcription) were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play central roles in interferon IFNα- and IFNγ-regulated gene expression. Western analysis of the full length STAT5b protein isolated from a variety of cultured cells, indicates that this protein migrates with an apparent molecular weight of ~92kDa. Validated applications for mouse anti-STAT5 Beta monoclonal antibody are Western blotting, immunoprecipitation, electrophoretic mobility shift assay (EMSA) and ELISA.
Applications: Validated applications for STAT5 Beta monoclonal antibody are Western blotting, immunoprecipitation, electrophoretic mobility shift assay (EMSA) and ELISA.
Host species and isotype: The host species and isotype of the STAT5 Beta monoclonal antibody is mouse IgG2a lambda.
Clone ID of monoclonal antibody (mAb): The mouse anti-STAT5 Beta monoclonal antibody clone is ST5b-10G1.
Reactivity: Mouse anti-STAT5 Beta monoclonal antibody detects human, mouse and rat STAT5 Beta.
Product size: Mouse anti-STAT5 Beta monoclonal antibody is available in a 100 µg pack size.
STATs (signal transducers and activators of transcription) were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play central roles in interferon IFNα- and IFNγ-regulated gene expression. Following the identification of these first two family members, five additional mammalian STAT proteins have been cloned, and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6. More recently a putative Drosophila STAT protein termed D-STAT or Marelle was described.
In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding which results in transcriptional activation of distinct target genes. At least one and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members. Nevertheless, a striking specificity of specific STAT activation is seen in response to individual cytokines.
STAT5 or MGF (mammary growth factor) was originally isolated by purification of tyrosine phosphorylated DNA binding proteins from prolactin stimulated mammary tissue and from IL-3 stimulated myeloid cells. Subsequently, two highly related but distinct Stat5 genes (Stat5a and Stat5b) were identified in mouse. The amino acid sequences of STAT5a and 5b show ~ 96% sequence similarity, and both proteins are co-expressed in most tissues of both virgin and lactating mice. However, differential accumulation of 5a and 5b mRNAs have been reported for both muscle and mammary tissue. STAT5 has been reported to be activated by multiple cytokines including, IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GM-CSF, TPO, EPO, GH, and Prolactin (PRL). Both the STAT5a and 5b proteins recognize the GAS (gamma interferoin activating sequence) site TTCNNNGAA, and the preferred DNA-binding consensus site has been identified as TTCC (A>T) GGAA. Western analysis of the full-length STAT5b protein isolated from a variety of cultured cells, indicates that this protein migrates with an apparent molecular weight of ~92 kDa. Carboxy-terminal truncated forms of the STAT5b protein have also been reported.