Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines.

Features of Gibco® Sf-900™ III SFM:

• Superior long-term, high density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin-free ready-to-use formulation
• Improved lot-to-lot consistency

Superior Long-Term, High Density Growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 10⁶ cells/ml, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see Figure 1).

Optimized for Recombinant Protein Production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

Serum-Free, Protein-Free, Animal Origin-Free, Ready-to-Use Formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin-free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

Improved Lot-to-Lot Consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see Figure 1).

Universal Bag Packaging
Gibco® universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

cGMP Manufacturing and Quality System
Gibco® Sf-900 II SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.