SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity
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The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase offers the highest sensitivity available from Life Technologies for end point detection. This system combines the high thermostability of SuperScript® III RT with the specificity of Platinum® Taq DNA Polymerase High Fidelity to increase priming specificity, generate higher product yields, and identify a larger range of targets. The one-step format enables easy analysis of gene expression or detection of rare or viral RNA. • Sensitivity - Routine detection down to 1 pg total RNA • Convenience - Single-tube format for speed, convenience, and less reaction-to-reaction variability. Compatible with multiplexing • Specificity - Uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers • Amplicon Size - Detection of targets from 300 bp to 10 kb for greater flexibility (Figure 1)
High-Fidelity PCR Amplification Platinum® Taq DNA Polymerase High Fidelity is ideal for the amplification of DNA fragments, when high yields and robust amplification are required. Fidelity is maximized through the use of a mixture of Platinum® Taq DNA Polymerase and the proofreading (3’-5’ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
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