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Platinum® PCR SuperMix High Fidelity is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1).
Platinum® PCR SuperMix High Fidelity is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room temperature reaction assembly. With Platinum® PCR SuperMix High Fidelity you'll: -Achieve greater than six-times higher fidelity than Taq DNA polymerase -Amplify fragments up to 15 kb -Produce products that are mixed blunt and 3-A' ends; however, the majority of ends have a 3-A' overhang -Minimize PCR optimization
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