Platinum® PCR SuperMix High Fidelity is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room temperature reaction assembly.
With Platinum® PCR SuperMix High Fidelity you'll:
- Achieve greater than six-times higher fidelity than Taq DNA polymerase
- Amplify fragments up to 15 kb
- Produce products that are mixed blunt and 3-A' ends; however, the majority of ends have a 3-A' overhang
- Minimize PCR optimization