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Pfx50™ DNA Polymerase is a designer enzyme derived from the archaean Thermococcus zilligii. In addition to 3´ to 5´ proofreading ability, Pfx50™polymerase is fused to an accessory protein for enhanced fidelity and processivity. This unique combination makes the Pfx50™enzyme ideal for applications that require the highest fidelity and PCR success the first time. With Pfx50™DNA Polymerase, you will:
• Achieve ultra low error rates—50 times better than Taq, without compromising PCR robustness • Save time and money—highest fidelity means youll sequence fewer clones to obtain error-free constructs • Produce results quickly and easily—robust performance with extremely short extension holds
Downstream compatibility with popular cloning techniques Pfx50™DNA Polymerase produces blunt-ended PCR products for compatibility with Directional TOPO® Expression Kits and Zero Blunt® TOPO® PCR Cloning Kits.
Unit Definition: One unit of Pfx50™DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min at 74°C.
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