The Cloned AMV First-Strand Synthesis Kit combines the high performance of Invitrogen™ Cloned AMV RT with the convenience of optimized, pre-qualified reagents. The and ! subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen™ Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum® Taq DNA Polymerase for high-specificity PCR or with Platinum® Taq DNA Polymerase High Fidelity for subsequent PCR cloning.
The Cloned AMV First-Strand cDNA Synthesis Kit:

• Uses high-purity Cloned AMV for higher yields

• Includes 5X first-strand buffer with proprietary agents for more robust performance

• Replaces the cDNA Cycle® Kit and AMV Reverse

Transcriptase System

• Detects transcripts in 1.0 pg of RNA (Figure 1)

• Detects various mRNA targets ranging in size from 1.6 kb to 9.4 kb (Figure 2)