AMV Reverse Transcriptase, Cloned, is used to synthesize first-strand cDNA for amplification by PCR or for cloning subsequent to conversion to double-stranded cDNA. Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV. The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb. AMV RT has endoribonuclease H activity.

Features of this enzyme:

• Flexibility—works with oligo(dT), random primers, or gene-specific primers
• Thermostability—AMV is active at temperatures up to 55°C
• Multiple applications—first-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA

Source
Purified from insect cells infected with recombinant baculovirus expressing AMV pol.

Unit definition
One unit of cloned AMV RT is the amount of enzyme required to incorporate 1 nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)₂₅ as template-primer.