Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity.
• Flexibility—Works with oligo(dT), random primers, or a gene-specific primers
• Thermostability—AMV is active at temperatures up to 55°C
• Multiple Applications—First-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA

Source: Purified from insect cells infected with recombinant baculovirus expressing AMV pol.

Unit Definition: One unit of cloned AMV RT is the amount of enzyme required to incorporate 1 nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)
• oligo(dT)₂₅ as template
• primer (3).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.