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Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity. • Flexibility—Works with oligo(dT), random primers, or a gene-specific primers • Thermostability—AMV is active at temperatures up to 55°C • Multiple Applications—First-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNA
Source: Purified from insect cells infected with recombinant baculovirus expressing AMV pol.
Unit Definition: One unit of cloned AMV RT is the amount of enzyme required to incorporate 1 nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A) • oligo(dT)25 as template • primer (3).
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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