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Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly-specific, non-toxic fashion.
Oligofectamine™ offers superior performance because of the delivery mechanism results in highly specific activity (knockdown of target gene) with low non-specific effects (Figure 1). Oligofectamine™ is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3 and HeLa.
Oligofectamine™ Reagent is easy-to-use because it provides a simple and fast protocol. Just dilute Oligofectamine™ Reagent, mix with oligonucleotide, and add to your cells. Oligofectamine™ requires nanomolar quantities of antisense oligonucleotide, reducing the amount of valuable oligonucleotide needed by up to a thousand-fold. This makes it ideal for high-throughput applications.
In addition, Oligofectamine™ has also been shown to work for siRNA transfections. We recommend this reagent when performing RNAi knockdown experiments in HeLa cells. Please visit RNAi Central for more information.
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