The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A+
selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis.Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include:
—Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
—SuperScript® II is an enzyme you can trust for high-yield, high-quality, general-purpose first-strand synthesis.Simple, Reliable, and Trouble-Free
The SuperScript® Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript® Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50 µg total RNA, or 0.2–5 µg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, Life Technologies recommends the use of a high-quality RNA substrate prepared using TRIzol® RNA Isolation Reagents
For research use only. Not for use in diagnostic procedures.