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Next-generation, real-time qRT-PCR reagents designed to complement fast-cycling instruments Get the turnaround time you want with the data quality you need
EXPRESS One-Step SuperScript® qRT-PCR SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. These kits provide:
• Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase - complete, rapid activation provides the best sensitivity and reproducibility (Figure 1) • The One-Step SuperScript® III Reverse Transcriptase module has a unique formulation to ensure better yield and less PCR inhibition, specifically for one-step qRT-PCR • Flexible formats with ROX premixed or separate - designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™) (Figure 2) • The only commercially available one-step qRT-PCR kits containing heat-labile UDG - significantly reduced carryover contamination risk with excellent activity from room temperature to approximately 40·C. Due to the thermostability of SuperScript® III, the cDNA synthesis step is run at 50·C, at which temperature the heat-labile UDG is totally and irreversibly inactivated (Figure 3)
Easy to test and switch
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience. The kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs.
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