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EXPRESS One-Step SYBR® GreenER™ SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. These kits provide:
• Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase in association with the brighter and less PCR-inhibitory SYBR® GreenER™ dye - complete, rapid activation provides the best sensitivity and reproducibility (Figure 1) • The One-Step SuperScript™III Reverse Transcriptase module has a unique formulation to ensure better yield and less qPCR inhibition specifically for one-step qRT-PCR • Flexible format with ROX premixed or separate - designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™) (Figure 1) • Peace of mind with the only commercially available one-step qRT-PCR kits containing heat-labile UDG - significantly reduces carryover contamination risk thanks to its excellent activity from room temperature to approximately 40·C. Due to the thermostability of SuperScript® III Reverse Transcriptase, the cDNA synthesis step is run at 50·C, at which temperature the heat-labile UDG is totally and irreversibly inactivated (Figure 2).
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EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience. Additionally, kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs. This "Universal" kit contains a separate tube of ROX.
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