- Sensitive—Linearity from 1 pg - 2.5 µg in a 20 µl reaction
- Convenient—Single tube format, just add your RNA and go
- Flexible—Suitable for qPCR detection methods such as SYBR® Green and TaqMan® Assays
- Powerful—High yields of cDNA perfect for archiving
The SuperScript® VILO™ Master Mix provides the high-temperature capability of SuperScript® III Reverse Transcriptase in an optimized format. The SuperScript® VILO™ Master Mix can generate first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR) with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20- µL reaction).
Reliable, RNA Protected, Reverse Transcripition from Total RNA
Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. The SuperScript® VILO™ Master Mix also includes RNaseOUT™ Recombinant Ribonuclease Inhibitor to safeguard against the degradation of target RNA due to ribonuclease contamination. Robust SuperScript® III RT can be used to synthesize cDNA at a temperature range of 42–55°C.
Broad Linearity for Less Template Dilutions
Obtain the same relative representation in your cDNA qPCR template, regardless of gene abundance. During normalization, get both your gene of interest and the reference gene in a linear phase for cDNA synthesis even if their abundances differ in the starting material.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.