The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.Features of the CellsDirect™ One-Step qRT-PCR Kit include:
—Offers significant time and cost savings for increased throughput
—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq
—No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range
—Detect targets from samples ranging from a single cell to 10,000 cells
—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.Ideal for Your qRT-PCR Application
CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan®) or LUX™ fluorogenic primers.Note:
This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit (11754-100
), with 2X Reaction Mix containing ROX™ Reference Dye, is also available.