Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCR_x Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCR_x Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

Platinum® Pfx provides:

• 26 times higher fidelity than Taq DNA polymerase (1)

• Amplification of fragments up to 12 kb

• Room temperature reaction assembly

Application: Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR (1). Platinum® Pfx is especially effective where the very high levels of fidelity are required.

Unit Definition: One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min. at 74°C.