Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Life Technologies offers a variety of Gibco® Media options for a range of insect cell culture applications.
The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual Site cGMP Manufacturing and Quality System
Gibco® Grace's Supplemented Insect Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® Grace's Supplemented Insect Medium made in our Scotland facility (11605-086). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO₂ in order to maintain physiological pH.